8D1E
hBest2 1uM Ca2+ (Ca2+-bound) closed state
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 1.78 |
Number of Particle | 2040714 |
Software | cryoSPARC,PHENIX,Coot,REFMAC,Leginon |
Symmetry | C5 |
CTF correction | NONE |