8D1E
hBest2 1uM Ca2+ (Ca2+-bound) closed state
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 1.78 |
| Number of Particle | 2040714 |
| Software | cryoSPARC,PHENIX,Coot,REFMAC,Leginon |
| Symmetry | C5 |
| CTF correction | NONE |
