8D0R

Human FUT9 bound to GDP and H-Type 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	127.690, 127.690, 127.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.379 - 1.400
R-factor	0.1512
Rwork	0.150
R-free	0.16840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8d0o
RMSD bond length	0.005
RMSD bond angle	0.874
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	1.440
High resolution limit [Å]	1.400	1.400
Rmeas	0.066	2.300
Number of reflections	67782	6759
<I/σ(I)>	19.1	1.4
Completeness [%]	99.9	100
Redundancy	15.1	15.3
CC(1/2)	1.000	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.6 M Ammonium sulfate, 100 mM Tris pH 8.0