8D0Q

Human FUT9 bound to GDP-CF3-Fucose and H-Type 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	127.810, 127.810, 127.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.900 - 1.390
R-factor	0.153
Rwork	0.152
R-free	0.17200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8d0o
RMSD bond length	0.008
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	1.430
High resolution limit [Å]	1.390	1.390
Rmeas	0.050	1.860
Number of reflections	69390	6880
<I/σ(I)>	26.3	1.4
Completeness [%]	99.9	100
Redundancy	17	12
CC(1/2)	1.000	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.6 M Ammonium sulfate, 100 mM Tris pH 8.0