8D0O
Human alpha1,3-fucosyltransferase FUT9, heavy atom derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-02 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.7 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 127.120, 127.120, 127.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.900 - 2.100 |
| R-factor | 0.1665 |
| Rwork | 0.164 |
| R-free | 0.20880 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.900 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.093 | 0.450 |
| Number of reflections | 19803 | 1771 |
| <I/σ(I)> | 42.4 | 6.4 |
| Completeness [%] | 99.1 | 88.3 |
| Redundancy | 43 | 15 |
| CC(1/2) | 1.000 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6 M Ammonium sulfate, 100 mM Tris pH 8.0 |
