8CYN
[2T7+20bp Linker] Self-Assembled 3D DNA Hexagonal Tensegrity Triangle with 20 bp Sticky-End Linker
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-13 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.00743 |
Spacegroup name | P 63 |
Unit cell lengths | 231.034, 231.034, 100.851 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.020 - 9.450 |
R-factor | 0.0975 |
Rwork | 0.096 |
R-free | 0.14400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7r96 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.762 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.060 | 10.850 |
High resolution limit [Å] | 9.450 | 9.450 |
Number of reflections | 1358 | 194 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 86.1 | |
Redundancy | 18 | |
CC(1/2) | 0.999 | 0.672 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.75 M ammonium sulfate, 120 mM Tris, 120 mM Acetic Acid, 6 mM EDTA |