7R96

Self-Assembled 3D DNA Hexagonal Tensegrity Triangle

Summary for 7R96

• Entry DOI: 10.2210/pdb7r96/pdb
• Descriptor: DNA (5'-D(*AP*GP*GP*CP*AP*GP*CP*CP*TP*GP*TP*AP*CP*GP*GP*AP*CP*AP*TP*CP*A)-3'), DNA (5'-D(P*CP*CP*GP*TP*AP*CP*A)-3'), DNA (5'-D(P*GP*GP*CP*TP*GP*C)-3'), ... (4 entities in total)
• Functional Keywords: tensegrity triangle, synthetic construct, self-assembly, dna
• Biological source: synthetic construct; More
• Total number of polymer chains: 4
• Total formula weight: 12797.42

Authors

Lu, B.,Vecchioni, S.,Ohayon, Y.P.,Sha, R.,Mao, C.,Seeman, N.C. (deposition date: 2021-06-28, release date: 2021-10-20, Last modification date: 2023-10-18)

Primary citation

Lu, B.,Vecchioni, S.,Ohayon, Y.P.,Sha, R.,Woloszyn, K.,Yang, B.,Mao, C.,Seeman, N.C.
3D Hexagonal Arrangement of DNA Tensegrity Triangles.
Acs Nano, 15:16788-16793, 2021

PubMed Abstract: The tensegrity triangle motif utilizes Watson-Crick sticky end cohesion to self-assemble into a rhombohedral crystal lattice using complementary 5'-GA and 5'-TC sticky ends. Here, we report that using noncanonical 5'-AG and 5'-TC sticky ends in otherwise isomorphic tensegrity triangles results in crystal self-assembly in the 6 hexagonal space group as revealed by X-ray crystallography. In this structure, the DNA double helices bend at the crossover positions, a feature that was not observed in the original design. Instead of propagating linearly, the tilt between base pairs of each right-handed helix results in a left-handed superstructure along the screw axis, forming a microtubule-like structure composed of three double helices with an unbroken channel at the center. This hexagonal lattice has a cavity diameter of 11 nm and a unit cell volume of 886 000 Å-far larger than the rhombohedral counterpart (5 nm, 330 000 Å).

PubMed: 34609128
DOI: 10.1021/acsnano.1c06963

Experimental method

X-RAY DIFFRACTION (5.68 Å)