8CVK

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	210.160, 452.740, 623.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	128.400 - 2.500
R-factor	0.2377
Rwork	0.235
R-free	0.28440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.851
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	128.400	128.400	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.187	0.051	1.507
Rmeas	0.205	0.056	1.684
Total number of observations	11647854
Number of reflections	2012356	23501	146758
<I/σ(I)>	7.99	23.6	0.93
Completeness [%]	99.6	98.7	98.6
Redundancy	5.788	6.927	4.881
CC(1/2)	0.986	0.996	0.194

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME