8CUK

X-ray Structure of the WD40 domain of HOPS subunit Vps11 from Yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.743
Spacegroup name	P 21 21 21
Unit cell lengths	89.084, 113.159, 129.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.760 - 2.220
R-factor	0.1773
Rwork	0.174
R-free	0.23410
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.893
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXCD
Refinement software	PHENIX (1.17-3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.260
High resolution limit [Å]	2.220	6.020	2.220
Rmerge	0.090	0.032	0.928
Total number of observations	432332
Number of reflections	62899	3503	2741
<I/σ(I)>	7.5
Completeness [%]	96.2	99.6	85.5
Redundancy	6.9	7.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris pH 7.5, 11% PEG 6000, 3% glycerol, 0.5 mM TCEP. Cryoprotected with 30% glycerol supplementing the crystallization conditions.