8CUA

Human excitatory amino acid transporter 3 (EAAT3) with bound potassium in an intermediate outward facing state

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.44
Number of Particle	3502663
Software	RELION,PHENIX,cryoSPARC,Leginon,CTFFIND,UCSF ChimeraX,Coot
Symmetry	C3
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION