8CT5
Catalytic Core Domain of HIV-1 Integrase (F185K)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-13 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.92 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.973, 72.973, 66.097 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.300 - 1.970 |
R-factor | 0.20435 |
Rwork | 0.201 |
R-free | 0.23648 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l3u |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.300 | 2.044 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.018 | 0.151 |
Rmeas | 0.026 | 0.213 |
Rpim | 0.018 | 0.151 |
Number of reflections | 14661 | 1422 |
<I/σ(I)> | 27.3 | 3.6 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 2 | 2 |
CC(1/2) | 0.999 | 0.942 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 7% PEG 8000, 0.2 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.5, 5 mM manganese chloride, 5 mM magnesium chloride, and 5 mM DTT |