8CSG
Human PRMT5:MEP50 structure with Fragment 1 and MTA Bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99986 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 100.662, 137.181, 177.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.630 - 2.480 |
| Rwork | 0.263 |
| R-free | 0.29160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.609 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.630 | 2.730 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.440 | |
| Number of reflections | 42080 | 10721 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 95.7 | 98.2 |
| Redundancy | 2.8 | 2.8 |
| CC(1/2) | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 13% PEG 4000, 100 mM Sodium Acetate pH 5.0 |
