8CS5

[GGA/CTC] Self-Assembled 3D DNA Hexagonal Tensegrity Triangle

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 63
Unit cell lengths	123.204, 123.204, 57.076
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.330 - 6.520
R-factor	0.2206
Rwork	0.219
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7r96
RMSD bond length	0.008
RMSD bond angle	1.065
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.350	7.280
High resolution limit [Å]	6.520	6.520
Number of reflections	928	182
<I/σ(I)>	12.5
Completeness [%]	90.9
Redundancy	19.3
CC(1/2)	0.999	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	1.75 M ammonium sulfate, 120 mM Tris, 120 mM Acetic Acid, 6 mM EDTA