8CR8

human Interleukin-23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	67.748, 59.842, 69.989
Unit cell angles	90.00, 98.06, 90.00

Refinement procedure

Resolution	69.300 - 2.000
R-factor	0.2362
Rwork	0.235
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.525
Data reduction software	XDS (20200131)
Data scaling software	XDS (20200131)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.300	2.450	2.120
High resolution limit [Å]	2.000	2.270	2.000
Rmeas	0.073	0.558	1.411
Number of reflections	35521	5252	4469
<I/σ(I)>	13.9	3.41	1.02
Completeness [%]	94.3	99.3	74.1
Redundancy	6.58	7.05	5.07
CC(1/2)	0.999	0.898	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287	(Morpheus2 E7) 2mM Lanthanides, 0.1M BES/ TEA, 10% PEG8000,20% 1.5-pentanediol