8CQZ

Homo sapiens Get3 in complex with the Get1 cytoplasmic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972
Spacegroup name	P 21 21 21
Unit cell lengths	69.478, 81.549, 92.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.900 - 2.800
R-factor	0.2466
Rwork	0.242
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.472
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (v1.15)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.045	1.579
Rpim	0.018	0.448
Number of reflections	13414	1324
<I/σ(I)>	25	1.4
Completeness [%]	96.5
Redundancy	12.8
CC(1/2)	0.999	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	44 % (v/v) 1,2-propanediol, 0.05 M calcium acetate and 0.1 M sodium acetate (pH 5.0)