8CQW

Crystal structure of the Candida albicans 80S ribosome in complex with Hygromycin B

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	173
Detector technology	PIXEL
Collection date	2022-03-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	298.810, 292.840, 450.180
Unit cell angles	90.00, 100.11, 90.00

Refinement procedure

Resolution	163.720 - 3.050
R-factor	0.2828
Rwork	0.283
R-free	0.30680
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.781
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	PHENIX ((1.19rc4_4035: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	163.720	163.720	3.100
High resolution limit [Å]	3.050	50.000	3.050
Rmerge	0.838	0.052	2.659
Rmeas	0.868	0.054	2.754
Rpim	0.226
Number of reflections	1429361	282	67955
<I/σ(I)>	3.69
Completeness [%]	99.1
Redundancy	14.5
CC(1/2)	0.986	0.999	0.044

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	PEG 20K, KSCN, Mg Acetate, K - acetate, Bis-Tris-Acetate, Glycerol, Spermidine, phyllanthoside