8COE

complement C5 in complex with the LCP0195 nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	I 41
Unit cell lengths	194.830, 194.830, 207.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.710 - 4.200
R-factor	0.2493
Rwork	0.248
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.402
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.710	4.350
High resolution limit [Å]	4.200	4.200
Rmerge	0.129	2.421
Number of reflections	28237	2779
<I/σ(I)>	11.94
Completeness [%]	99.8	100
Redundancy	14.1	14.2
CC(1/2)	0.999	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.025 M succinic acid pH 7.0 and 3.75% w/v polyethylene glycol 3350.