8COD
Crystal structure of S-adenosyl-L-homocysteine hydrolase from Mus musculus in complex with inosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 98.220, 102.526, 173.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 88.250 - 2.480 |
R-factor | 0.14936 |
Rwork | 0.146 |
R-free | 0.21625 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.568 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.250 | 2.520 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.180 | 0.970 |
Number of reflections | 31056 | 1520 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 98.8 | |
Redundancy | 5.7 | |
CC(1/2) | 0.990 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 180 mM sodium formate, pH 6.9, and 22% PEG 3350 |