8CN0

Crystal structure of CREBBP-Y1482N histone acetyltransferase domain in complex with Coenzyme A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98400
Spacegroup name	I 2 2 2
Unit cell lengths	87.009, 148.586, 155.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.887 - 2.440
R-factor	0.2608
Rwork	0.258
R-free	0.30410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.028
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.887	2.671
High resolution limit [Å]	2.440	2.440
Rmerge	0.165	1.705
Rmeas	0.172	1.788
Rpim	0.047	0.528
Number of reflections	23398	1170
<I/σ(I)>	14	1.6
Completeness [%]	94.4
Redundancy	13.3
CC(1/2)	0.997	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0,2 M Potassium citrate tribasic monohydrate 20 % w/v Polyethylene glycol 3,350 pH 8,2