8CIR
The FERM domain of human moesin with a bound peptide identified by phage display
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-14 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.466, 155.391, 63.607 |
Unit cell angles | 90.00, 94.28, 90.00 |
Refinement procedure
Resolution | 77.696 - 1.850 |
Rwork | 0.178 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.635 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.700 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 74812 | 4621 |
<I/σ(I)> | 7.8 | 0.9 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 13.2 | |
CC(1/2) | 0.997 | 0.347 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM sodium bromide, 100 mM bis-tris-propane, 10% ethylene glycol, 20% PEG3350 |