8CIO
Cryo-EM structure of the CupE pilus from Pseudomonas aeruginosa
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.5 |
| Number of Particle | 3766858 |
| Software | RELION,PHENIX,EPU,CTFFIND,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
