8CGE
The crystal structure of a cobalt-bound scFv reveals a Tetrameric polyHistidine motif (TetrHis)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9990 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 97.496, 81.817, 111.045 |
| Unit cell angles | 90.00, 111.14, 90.00 |
Refinement procedure
| Resolution | 45.170 - 2.500 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.318 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.470 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 28177 | 635 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.989 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.01M cobalt chloride, 0.1M MES, pH6.5, 1.8 M ammonium sulfate |
