8CE6

Crystal structure of human Cd11b I domain in P212121 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-08
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	39.482, 50.906, 101.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.700 - 1.582
Rwork	0.175
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.685
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.700	1.696
High resolution limit [Å]	1.575	1.575
Number of reflections	26352	1133
<I/σ(I)>	16.5
Completeness [%]	91.5
Redundancy	11.7
CC(1/2)	0.999	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15M ammonium sulfate 25% PEG4000 15% glycerol