8CE0

N-terminal domain of human apolipoprotein E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999
Spacegroup name	P 21 21 21
Unit cell lengths	45.529, 53.085, 73.171
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.657 - 1.750
R-factor	0.1915
Rwork	0.190
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.227
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.530	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	18525	981
<I/σ(I)>	16.4
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	1.000	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	NaCl, Hepes, PEG 8000