8CDS
Crystal structure of the xhNup93-Nb4i VHH antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.877, 42.105, 44.946 |
Unit cell angles | 90.00, 93.06, 90.00 |
Refinement procedure
Resolution | 30.710 - 1.530 |
R-factor | 0.1376 |
Rwork | 0.133 |
R-free | 0.17550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.804 |
Data reduction software | XDS (Jan 31, 2020 BUILT=20200417) |
Data scaling software | XSCALE (Jan 31, 2020 BUILT=20200417) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.710 | 30.710 | 1.440 |
High resolution limit [Å] | 1.360 | 4.060 | 1.360 |
Rmerge | 0.053 | 0.040 | 2.489 |
Rmeas | 0.064 | 0.047 | 3.071 |
Number of reflections | 40137 | 1594 | 6289 |
<I/σ(I)> | 9.94 | ||
Completeness [%] | 94.5 | ||
Redundancy | 6.2187 | ||
CC(1/2) | 0.997 | 0.997 | 0.312 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.1 M imidazole pH 8, 30% (w/v) PEG 8,000, 0.2 M NaCl |