8CDG
Crystal structure of human IL-17A cytokine in complex with macrocycle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.362, 89.771, 116.669 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.390 - 2.900 |
R-factor | 0.2348 |
Rwork | 0.233 |
R-free | 0.25690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.535 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.390 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.068 | |
Number of reflections | 8077 | 591 |
<I/σ(I)> | 22.5 | |
Completeness [%] | 99.5 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 100 mM sodium citrate buffer, pH 5.5, 8 % 2-propanol, and 10 % w/v PEG10k |