8CBF
SARS-CoV-2 Delta-RBD complexed with Omi-42 and Beta-49 Fabs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-19 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 177.002, 144.275, 49.665 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.140 - 2.330 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.613 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.370 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.300 | |
| Rpim | 0.086 | |
| Number of reflections | 55255 | 2547 |
| <I/σ(I)> | 7.1 | 0.4 |
| Completeness [%] | 99.8 | 93.3 |
| Redundancy | 13 | 8.7 |
| CC(1/2) | 0.998 | 0.332 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | containing 0.1 M sodium citrate tribasic dihydrate pH 5.5 and 22% (w/v) PEG 1000 |
