8C6I
TMEM2 ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-03 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 184.760, 184.760, 105.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.550 - 3.500 |
| R-factor | 0.2678 |
| Rwork | 0.265 |
| R-free | 0.31890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.638 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.350 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rpim | 0.073 | 0.871 |
| Number of reflections | 23570 | 1159 |
| <I/σ(I)> | 7.1 | 0.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 26.8 | |
| CC(1/2) | 0.997 | 0.424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium acetate, pH 4.5, 30% polyethyleneglycol 3000 |
