8C5L

NR2F6 ligand binding domain in complex with NSD1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972425
Spacegroup name	P 61
Unit cell lengths	170.383, 170.383, 83.667
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.340 - 2.600
R-factor	0.202
Rwork	0.200
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.895
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0.352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	147.560	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	42757	4485
<I/σ(I)>	11.7
Completeness [%]	100.0	100
Redundancy	9.6	10.1
CC(1/2)	0.999	0.376

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	90 mM SPG pH 8.0, 22.5% w/v PEG1500, 100 mM potassium chloride