8C5L
NR2F6 ligand binding domain in complex with NSD1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972425 |
| Spacegroup name | P 61 |
| Unit cell lengths | 170.383, 170.383, 83.667 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.340 - 2.600 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.895 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0.352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.560 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 42757 | 4485 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 10.1 |
| CC(1/2) | 0.999 | 0.376 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 90 mM SPG pH 8.0, 22.5% w/v PEG1500, 100 mM potassium chloride |
