8C1L
Crystal structure of HNF4 alpha LBD in complexes with palmitic acid and GRIP-1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.277, 82.457, 57.360 |
| Unit cell angles | 90.00, 111.84, 90.00 |
Refinement procedure
| Resolution | 47.520 - 2.000 |
| R-factor | 0.19733 |
| Rwork | 0.195 |
| R-free | 0.23667 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.197 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.370 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 32417 | 2491 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 97.0 | |
| Redundancy | 5.2 | |
| CC(1/2) | 0.999 | 0.822 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 284 | 20% PEG3350 -- 10% ethylene glycol -- 0.1M bis-tris-propane pH 8.5 -- 0.2M sodium nitrate |
