EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.9 |
Number of Particle | 215225 |
Software | crYOLO,ISOLDE,RELION,EPU,Gctf,Coot,PHENIX |
Symmetry | I |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |