8BZS
The crystal structure of glycogen phosphorylase in complex with baicalein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-11-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.510, 128.510, 116.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.600 - 2.250 |
| R-factor | 0.15357 |
| Rwork | 0.151 |
| R-free | 0.20261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r0h |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.815 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.600 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.132 | |
| Number of reflections | 45448 | 3980 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 97.5 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.984 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.8 | 289 | 10 mM BES buffer |
