8BYU
Crystal Structure of HexaBody-CD38 Fab in complex with CD38
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.240, 78.309, 98.162 |
Unit cell angles | 90.00, 91.94, 90.00 |
Refinement procedure
Resolution | 61.000 - 1.850 |
R-factor | 0.1896 |
Rwork | 0.188 |
R-free | 0.22976 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yh3 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.343 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.000 | 1.880 |
High resolution limit [Å] | 1.848 | 1.848 |
Rmerge | 0.099 | |
Rmeas | 0.126 | |
Number of reflections | 63099 | 2880 |
<I/σ(I)> | 6.9 | |
Completeness [%] | 98.7 | |
Redundancy | 3 | |
CC(1/2) | 0.994 | 0.589 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.15 M (NH4)2SO4; 0.1 M HEPES: pH:7.0; 20.0 %w/v PEG 4K |