8BYU
Crystal Structure of HexaBody-CD38 Fab in complex with CD38
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.240, 78.309, 98.162 |
| Unit cell angles | 90.00, 91.94, 90.00 |
Refinement procedure
| Resolution | 61.000 - 1.850 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.22976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yh3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.343 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.000 | 1.880 |
| High resolution limit [Å] | 1.848 | 1.848 |
| Rmerge | 0.099 | |
| Rmeas | 0.126 | |
| Number of reflections | 63099 | 2880 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3 | |
| CC(1/2) | 0.994 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.15 M (NH4)2SO4; 0.1 M HEPES: pH:7.0; 20.0 %w/v PEG 4K |
