8BYQ
RNA polymerase II pre-initiation complex with the proximal +1 nucleosome (PIC-Nuc10W)
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Quantum LS |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.1 |
Number of Particle | 4606320 |
Software | Warp,PHENIX,cryoSPARC,RELION,SerialEM,UCSF Chimera,ISOLDE |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |