8BXK
Ntaya virus methyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-08 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.541870 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.090, 110.060, 116.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.960 - 2.310 |
R-factor | 0.2025 |
Rwork | 0.201 |
R-free | 0.23480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mrk |
RMSD bond length | 0.003 |
RMSD bond angle | 0.489 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.960 | 2.390 |
High resolution limit [Å] | 2.310 | 2.310 |
Number of reflections | 27743 | 1077 |
<I/σ(I)> | 16.4 | 3.7 |
Completeness [%] | 87.2 | |
Redundancy | 6.3 | |
CC(1/2) | 0.998 | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 4.0 M Sodium formate |