8BWS
Structure of yeast RNA Polymerase III elongation complex at 3.3 A
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | TFS TALOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.2 |
Software | RELION,Gctf |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |