8BWP
Cryo-EM structure of nanodisc-reconstituted wildtype human MRP4 (in complex with methotrexate)
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.6 |
Number of Particle | 1317394 |
Software | cryoSPARC,PHENIX,EPU,UCSF ChimeraX,Coot,ISOLDE |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |