8BWI

Crystal structure of human Twisted gastrulation protein homolog 1 (TWSG1), crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.96862
Spacegroup name	P 64 2 2
Unit cell lengths	92.250, 92.250, 95.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.120 - 3.400
R-factor	0.2977
Rwork	0.295
R-free	0.34240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.679
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.080	3.490
High resolution limit [Å]	3.400	3.400
Rmerge	0.251	5.961
Rmeas	0.265	6.167
Rpim	0.069	1.551
Number of reflections	3626	254
<I/σ(I)>	8.2	0.6
Completeness [%]	99.4	98.5
Redundancy	15.6	15.4
CC(1/2)	0.996	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	1.0 M K/Na tartrate, 0.1 M MES pH 6.0