8BW8

Crystal structure of the dCNK-SAM-CRIC-PDZ/dHYP-SAM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 31
Unit cell lengths	131.907, 131.907, 71.465
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.950 - 2.100
R-factor	0.1899
Rwork	0.188
R-free	0.21880
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.470
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	HKL2Map
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	114.230	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.062
Rmeas	0.069
Number of reflections	81051	4475
<I/σ(I)>	14	1
Completeness [%]	99.8
Redundancy	5.2
CC(1/2)	0.999	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.1M Bis-Tris Propane pH 7.0, and 1.0M Ammonium tartrate dibasic