8BUX
Rab-binding domain of human MiniBAR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.388, 123.956, 38.068 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.580 - 1.860 |
R-factor | 0.1672 |
Rwork | 0.165 |
R-free | 0.21400 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.052 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | SHELXD |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.580 | 1.888 |
High resolution limit [Å] | 1.856 | 1.856 |
Number of reflections | 24856 | 4668 |
<I/σ(I)> | 10.6 | |
Completeness [%] | 99.5 | |
Redundancy | 3.6 | |
CC(1/2) | 0.996 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | PEG |