8BUR
Structure of DDB1 bound to DS50-engaged CDK12-cyclin K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-19 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 250.266, 250.266, 217.643 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 97.250 - 3.640 |
R-factor | 0.1916 |
Rwork | 0.189 |
R-free | 0.23370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.929 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 216.737 | 3.812 |
High resolution limit [Å] | 3.640 | 3.643 |
Number of reflections | 79836 | 3990 |
<I/σ(I)> | 7.9 | |
Completeness [%] | 96.1 | |
Redundancy | 21.4 | |
CC(1/2) | 0.993 | 0.273 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.42 M ammonium sulphate, 0.07 M HEPES, pH 7.0 |