8BU6

Structure of DDB1 bound to DS55-engaged CDK12-cyclin K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	249.272, 249.272, 220.107
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.680 - 3.450
R-factor	0.2194
Rwork	0.218
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	215.874	3.678
High resolution limit [Å]	3.450	3.450
Number of reflections	77151	3863
<I/σ(I)>	12.9
Completeness [%]	95.1
Redundancy	21
CC(1/2)	0.999	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.42 M ammonium sulphate, 0.07 M HEPES, pH 7.3