8BTT

Structure of human RTCB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-23
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	1.0332
Spacegroup name	I 4
Unit cell lengths	215.640, 215.640, 46.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.220 - 2.600
R-factor	0.202
Rwork	0.199
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold model for Q9Y3I0
RMSD bond length	0.003
RMSD bond angle	0.517
Data reduction software	XDS (20210205)
Data scaling software	Aimless (1.12.10)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.220	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.184
Rpim	0.077	0.875
Number of reflections	33777	4030
<I/σ(I)>	8.5	0.9
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.995	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	protein: 125 uM in 25 mM Tris pH 7.5, 200 mM NaCl, 10 % glycerol, 1mM TCEP, 2 mM manganese chloride reservoir: 0.1 M magnesium chloride, 0.1 M HEPES pH 7.0, 15 % PEG 4000 drop: 800 nl protein + 600 nl reservoir