8BS6

Crystal structure of YTHDF3 disulfide mutant (closed conformation)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	61.900, 61.900, 81.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.520 - 1.200
R-factor	0.1802
Rwork	0.179
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.830	1.270
High resolution limit [Å]	1.200	1.200
Number of reflections	55256	8867
<I/σ(I)>	16.83
Completeness [%]	100.0
Redundancy	8.36
CC(1/2)	0.999	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	2M Disodium malonate