8BS4

Crystal structure of YTHDF1 YTH domain dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	91.850, 91.850, 206.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.830 - 2.100
R-factor	0.2161
Rwork	0.214
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.926	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	31056	4849
<I/σ(I)>	27.77
Completeness [%]	99.8
Redundancy	16.13
CC(1/2)	1.000	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	1.8 M ammonium citrate, 100 mM sodium acetate