8BRC
Crystal structure of the adduct between human serum transferrin and cisplatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 136.340, 156.400, 107.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 102.770 - 3.170 |
| R-factor | 0.1808 |
| Rwork | 0.177 |
| R-free | 0.24316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x1b |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.770 | 3.220 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Number of reflections | 19869 | 969 |
| <I/σ(I)> | 13.7 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 13.5 |
| CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 15% w/v PEG 3350, 16% v/v glycerol, 8 mM disodium malonate, and 150 mM Na-PIPES pH 6.5 |
