8BMO
Structure of GroEL:GroES complex exhibiting ADP-conformation in trans ring obtained under the continuous turnover conditions
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | JEOL CRYO ARM 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Software | crYOLO,RELION,PHENIX,SerialEM,CTFFIND,Coot |
| Symmetry | C7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
