8BLJ

Crystal structure of the ligand-binding domain (LBD) of human iGluR Delta-1 (GluD1), apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.999
Spacegroup name	P 1 21 1
Unit cell lengths	86.290, 107.569, 98.559
Unit cell angles	90.00, 94.08, 90.00

Refinement procedure

Resolution	62.590 - 2.180
R-factor	0.2075
Rwork	0.206
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v3t
RMSD bond length	0.002
RMSD bond angle	0.411
Data reduction software	autoPROC (1.0.5 (20210420))
Data scaling software	autoPROC (1.0.5 (20210420))
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0270)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.310	2.390
High resolution limit [Å]	2.180	2.180
Rmerge	0.189	0.845
Rpim	0.110	0.477
Number of reflections	56271	1959
<I/σ(I)>	4.7	1.6
Completeness [%]	91.2	61.8
Redundancy	3.8	3.9
CC(1/2)	0.986	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	17% (w/v) polyethylene glycol (PEG) 20000, 100 mM Tris HCl, 100 mM MgCl2