8BLJ
Crystal structure of the ligand-binding domain (LBD) of human iGluR Delta-1 (GluD1), apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.290, 107.569, 98.559 |
Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
Resolution | 62.590 - 2.180 |
R-factor | 0.2075 |
Rwork | 0.206 |
R-free | 0.24370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v3t |
RMSD bond length | 0.002 |
RMSD bond angle | 0.411 |
Data reduction software | autoPROC (1.0.5 (20210420)) |
Data scaling software | autoPROC (1.0.5 (20210420)) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0270) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.310 | 2.390 |
High resolution limit [Å] | 2.180 | 2.180 |
Rmerge | 0.189 | 0.845 |
Rpim | 0.110 | 0.477 |
Number of reflections | 56271 | 1959 |
<I/σ(I)> | 4.7 | 1.6 |
Completeness [%] | 91.2 | 61.8 |
Redundancy | 3.8 | 3.9 |
CC(1/2) | 0.986 | 0.641 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 17% (w/v) polyethylene glycol (PEG) 20000, 100 mM Tris HCl, 100 mM MgCl2 |