8BJP
The N288D mutant cytoplasmic PAS domain of Geobacillus thermodenitrificans histidine kinase CitA
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97957, 0.9800, 0.97188 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.166, 49.295, 92.199 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.509 - 2.100 |
| Rwork | 0.195 |
| R-free | 0.23260 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.614 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.509 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.038 | 0.267 |
| Number of reflections | 12630 | 582 |
| <I/σ(I)> | 20.96 | |
| Completeness [%] | 92.1 | |
| Redundancy | 3.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.4M magnesium chloride, 0.1M Tris/HCl, pH 8.5, 20.5% PEG 8000 |
