8BJP

The N288D mutant cytoplasmic PAS domain of Geobacillus thermodenitrificans histidine kinase CitA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97957, 0.9800, 0.97188
Spacegroup name	P 21 21 21
Unit cell lengths	49.166, 49.295, 92.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.509 - 2.100
Rwork	0.195
R-free	0.23260
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.614
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.509	2.150
High resolution limit [Å]	2.100	2.100
Rmeas	0.038	0.267
Number of reflections	12630	582
<I/σ(I)>	20.96
Completeness [%]	92.1
Redundancy	3.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.4M magnesium chloride, 0.1M Tris/HCl, pH 8.5, 20.5% PEG 8000