8BJD

Full length structure of LpMIP with bound inhibitor JK095

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	76.752, 76.752, 103.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.240 - 2.400
Rwork	0.245
R-free	0.29430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fd9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.670	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.060	0.080
Number of reflections	19644	1218
<I/σ(I)>	11.1
Completeness [%]	99.9	99.75
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	15 %(w/v) PEG 6000, 500 mM zinc acetate dihydrate, 100 mM MES, pH 6.5.