8BIX
Cystathionine gamma-lyase N360S mutant from Toxoplasma gondii in complex with cystathionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 158.427, 158.427, 93.828 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.378 - 1.590 |
| R-factor | 0.1726 |
| Rwork | 0.172 |
| R-free | 0.19040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nmp |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.412 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.380 | 1.620 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 177039 | 7304 |
| <I/σ(I)> | 22.3 | |
| Completeness [%] | 97.7 | |
| Redundancy | 16.7 | |
| CC(1/2) | 0.999 | 0.576 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 12% (w/v) PEG 3350, 0.1M sodium citrate pH 4.6, n-octyl-beta-D-thiomaltoside |
